Table 3.
Example of data measured on a plate reader
CellTiter-glo values |
F-Luc values (background subtracted) |
Ratio F-Luc/CellTiter-Glo |
|||||||
---|---|---|---|---|---|---|---|---|---|
Transfected expression construct: | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 1 | Replicate 2 | Replicate 3 |
Vector (not-stimulated) | 1362230 | 1565560 | 1500490 | 1450 | 1510 | 1560 | 0.001064 | 0.000965 | 0.00104 |
Vector | 1331190 | 1428330 | 1679530 | 226560 | 238610 | 240170 | 0.170194 | 0.167055 | 0.142998 |
MeV V | 1204400 | 1302960 | 1520620 | 8810 | 9450 | 9480 | 0.007315 | 0.007253 | 0.006234 |
NSP1 | 1034860 | 1010910 | 1314860 | 2010 | 2460 | 3110 | 0.001942 | 0.002433 | 0.002365 |
NSP2 | 1350470 | 1279680 | 1480560 | 242850 | 321050 | 321780 | 0.179826 | 0.250883 | 0.217337 |
NSP4 | 1317270 | 1425710 | 1634980 | 263370 | 285120 | 259060 | 0.199936 | 0.199985 | 0.158448 |
NSP5 | 1329170 | 1224430 | 1341000 | 17710 | 17040 | 16590 | 0.013324 | 0.013917 | 0.012371 |
NSP6 | 1652360 | 1422720 | 1553980 | 288370 | 329320 | 329990 | 0.17452 | 0.231472 | 0.212352 |
NSP7 | 1485790 | 1281010 | 1371270 | 184350 | 174400 | 153450 | 0.124075 | 0.136143 | 0.111904 |
NSP8 | 1107500 | 1294790 | 1168750 | 223180 | 187600 | 198390 | 0.201517 | 0.144888 | 0.169745 |
NSP9 | 1300610 | 1216560 | 1300440 | 66540 | 70800 | 67780 | 0.051161 | 0.058197 | 0.052121 |
NSP10 | 1258980 | 1225790 | 1347270 | 35370 | 37350 | 28890 | 0.028094 | 0.03047 | 0.021443 |
NSP11 | 1354470 | 1081270 | 1292670 | 107890 | 101730 | 92780 | 0.079655 | 0.094084 | 0.071774 |
NSP12 | 1252870 | 1377740 | 1413930 | 298450 | 272720 | 250010 | 0.238213 | 0.197947 | 0.176819 |
NSP13 | 1403890 | 1353110 | 1470630 | 13100 | 11270 | 10640 | 0.009331 | 0.008329 | 0.007235 |
NSP14 | 864250 | 832900 | 879820 | 20220 | 15930 | 18500 | 0.023396 | 0.019126 | 0.021027 |
NSP15 | 1347210 | 1293850 | 1296670 | 105790 | 114850 | 117120 | 0.078525 | 0.088766 | 0.090324 |
S | 1140010 | 1223150 | 1051670 | 97460 | 116430 | 117480 | 0.08549 | 0.095189 | 0.111708 |
ORF3a | 890390 | 739520 | 851310 | 43040 | 49220 | 49530 | 0.048338 | 0.066557 | 0.058181 |
ORF3b | 1137650 | 1064850 | 963730 | 55500 | 56900 | 61940 | 0.048785 | 0.053435 | 0.064271 |
E | 953870 | 966710 | 970660 | 28070 | 31450 | 33820 | 0.029427 | 0.032533 | 0.034842 |
M | 774430 | 748550 | 866140 | 64730 | 74490 | 72170 | 0.083584 | 0.099512 | 0.083324 |
ORF6 | 1064100 | 993390 | 1031010 | 2650 | 2400 | 2630 | 0.00249 | 0.002416 | 0.002551 |
ORF7a | 1496400 | 1294270 | 1295250 | 43990 | 49550 | 44270 | 0.029397 | 0.038284 | 0.034179 |
ORF7b | 1178080 | 999530 | 945810 | 12360 | 12230 | 12120 | 0.010492 | 0.012236 | 0.012814 |
ORF8 | 1364570 | 1250760 | 1172740 | 33350 | 33980 | 29450 | 0.02444 | 0.027167 | 0.025112 |
N | 1544600 | 1432180 | 1280710 | 418770 | 430120 | 388390 | 0.271119 | 0.300325 | 0.303261 |
ORF9b | 1454850 | 1364790 | 1401950 | 155300 | 167400 | 152140 | 0.106746 | 0.122656 | 0.10852 |
ORF9c | 1500700 | 1289060 | 1153010 | 71230 | 68740 | 67010 | 0.047465 | 0.053326 | 0.058117 |
ORF10 | 1519340 | 1424720 | 1292400 | 86330 | 84610 | 76260 | 0.056821 | 0.059387 | 0.059006 |