Figure 2.

Tissue-Specific Expression of Cre Recombinase, Cas9, and GFP Does Not Impact Body Weight or Tissue Weights. All four tissue-specific Cas9 mouse lines were aged to approximately 18–20 weeks of age and analyzed for A. body weight, B. liver weight at cull, C. fat mass by EchoMRI (final two weeks), D. lean mass by EchoMRI (final 2 weeks) and E. heart weight at study end. The four groups per line are as follows: Cre (inactive––light grey), Cre+Cas9 (inactive––dark grey), Cre (active––light blue), and Cre+Cas9 (active––dark blue). All data are presented as mean ± SEM, n = 6–11/group. Albumin-Cre line is constitutively active, so the inactive groups were designated as “not inducible.”