Concentration and time response of human KHK-C recombinant protein
(A) Progression curves of KHK-C reaction measured at different incubation intervals and with different concentrations of recombinant human KHK-C protein (0–16 nM).
(B) Linearity of ADP production using increasing recombinant KHK-C protein concentrations at fixed reaction time.
(C) Assay signal stability post addition of ADP-Glo detection reagent.