Figure 2.

KHK-C expression and activity in cell culture and mouse tissue homogenates

(A and B) KHK-C protein (A) and activity (B) levels from WT and KHK-C OE HepG2 cells (n=3). The amount of KHK protein is normalized to actin loading control. KHK activity was measured using no fructose, 2 mM fructose, or 2 mM 3-O-methyl-d-fructose as substrate.

(C and D) KHK protein (C) and activity (D) from liver homogenates of mice fed LFD or HFD with KHK OE and KD. The amount of KHK protein was normalized to vinculin loading control (n=4 and 5).

∗p≤0.05; ∗∗∗p≤0.001; ∗∗∗∗p≤0.0001 via Student’s t-test. Error bars represent standard error of the mean.