Figure 3.

Effects of temperature and potassium content on KHK activity

(A) KHK activity was measured in liver homogenates of mice fed LFD and HFD treated with KHK siRNA in the presence or absence of 2 mM fructose.

(B and C) (B) KHK activity after 70°C heat inactivation for 5 min or (C) with different concentrations of potassium.

(D) Activity of recombinant human KHK-C measured in the presence of different concentrations of NaCl and KCl in different buffers (50 mM Tris-Cl, pH 7.5; 50 mM Hepes pH7.4 or 50 mM MOPS pH 7.2).

∗p≤0.05; ∗∗∗p≤0.001; and ∗∗∗∗P≤0.0001 via Student’s t-test. Error bars represent standard error of the mean.