2× substrate solution

Component	2× concentration	Volume (total 1 mL)
1× assay buffer	1×	Up to 1000 μL
ATP (10 mM)	200 uM	20 μL
Fructose or 3OMF (500 mM)	4 mM	8 μL
Aliquots of ATP can be stored at −20°C to −15°C for up to six months. Freezing/thawing should be avoided. Fructose and 3OMF can be stored at 4°C.