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. 2021 Jul 28;24(8):102910. doi: 10.1016/j.isci.2021.102910

Figure 3.

Figure 3

OspC3 binds to primed caspase-11, suppressing its activity

(A–D) HA-conjugated beads were incubated with lysates from HEK293T cells expressing OspC3-HA. HA-conjugated beads bound to OspC3-HA were mixed with lysates from HEK293T cell expressing the indicated caspases (A), with mock or lysates from caspase-11-expressing HEK293T cells IFN-γ (50 ng/mL) primed for 4 hr or not (B), with BMM lysates LPS primed or not (C), or with lysates from BMMs transfected with LPS (5 μg/mL) for 4 hr to activate the non-canonical inflammasome following IFN-γ priming or not (D). Western blots were probed with the indicated antibodies. GAPDH was used as a loading control.