Stop solution

Reagents	Stock concentration	Final concentration	Amount
HBSS	2×	1×	50 mL
CBS	n/a	10% (v/v)	10 mL
Penicillin/Streptomycin	100×	1×	1 mL
Total	Add deionized water and constant volume to 100 mL

Prepare in a sterile environment, always prepare freshly, and do not store.