Low glucose buffer

Reagents	Stock concentration	Final concentration	Amount
KRB	2×	1×	10 mL
BSA	10% (w/v)	0.02% (w/v)	400 μL
Glucose (dynamic test)	-	-	-
Glucose (static test)	50% (w/v)	3.3 mmol/l	23.7 μL
Total	Add deionized water and constant volume to 20 mL

Prepare in a sterile environment, always prepare freshly, and do not store.