Table 2.
An example of the calculation of the quantities of extracted sterol standards according to the peak areas obtained by the chromatographs in Figure 3
| TIC peak area | Extraction efficiency (%) | Quantity (μg) | Corrected quantity (μg) | |
|---|---|---|---|---|
| Coprostanol (Internal Standard) | 4549529 | 0.79 | ||
| Cholesterol | 7323398 | 0.12 | 0.15 | |
| Desmosterol | 5582508a | 0.94 | 1.19 | |
| 7-dehydrocholesterol | 2427466a | 0.14 | 0.18 | |
| Ergosterol | 1918794 | 0.13 | 0.17 | |
| Stigmasterol | 6304675 | 0.12 | 0.15 | |
| β-sitosterol | 3686069 | 0.09 | 0.12 |
The calculation of quantities is based on the peak area of each sterol and its calibration curve equation (Table 1), divided by the extraction efficiency of the internal standard.
a
Areas of desmosterol and 7-dehydrocholesterol were estimated as described below.