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. 2021 Jun 18;76(9):2317–2324. doi: 10.1093/jac/dkab199

Figure 1.

Figure 1.

Purified pyocins S5 and AP41 are active in vitro. (a) Spot tests to determine the activity of pyocins S5 and AP41 against P. aeruginosa strain P7; 5 μL of a range of concentrations of pyocin S5/AP41 was spotted on a growing lawn of P. aeruginosa. The presence of clear zones indicates pyocin activity. (b) Bacterial counts over time of P. aeruginosa incubated in LB medium supplemented with PBS (control) or 10 mg/L S5, AP41 or S5+AP41. (c) Bacterial counts of P. aeruginosa after 4 h of incubation with PBS, S5, AP41 or S5+AP41 (10 mg/L) in different conditions: LB, LB + 200 nM 2.2’ bipyridyl or LB+fresh whole murine blood (50%). Bars represent the average cfu counts in cfu/mL from three experiments. Error bars represent the SD and asterisks indicate significant differences, as assessed by one-way ANOVA (*P < 0.05).