Figure 4.

Pyocins S5 and AP41 have different dissemination and clearance profiles in mice. (a) Serum concentration of S5 and AP41 determined by ELISA following IV injection of 200 μg of pyocins in mice. Results represent the mean from three independent experiments, two mice per timepoint and per experiment. Error bars represent the SD. (b) Detection of pyocins S5 and AP41 in serum and organ homogenates at different timepoints (10 min, 30 min, 1 h, 3 h, 6 h, 9 h and 24 h) following injection using S5- or AP41-specific affinity-purified antibodies. Positive control (+) = 1.25 ng of purified S5 or AP41. Negative control (-) = corresponding homogenized organ from a mouse that did not receive injections of S5 or AP41. (c) Detection of pyocin S5 or AP41 activity in serum or homogenized organs at different timepoints following IV injection. Five microlitres of homogenized organ or serum was spotted on a growing lawn of P. aeruginosa strain P7. The presence of clear zones indicates pyocin activity.