Figure 8.

CD44 stain for participant 4 (female, age 50 years). (A) Baseline TFB; (B) 3 months TFB; (C) Baseline bland moisturizer; (D) 3 months bland moisturizer. There is more hyaluronan seen in the TFB arm (B) than in the bland moisturizer arm (D). (E) The images obtained after the CD44 immunohistochemical analysis were analyzed with ImageJ software. Five fields of view were assessed for each condition (at 10×). The mean intensity (in arbitrary units, AU) and standard deviation were determined for each condition. The values were compared with a Student’s t test; P < 0.05 was considered significant. CD44 expression significantly increased after both the bland moisturizer and TFB treatments. However, the intensity of CD44 after the TFB treatment was significantly greater than after the bland moisturizer treatment (168%; P < 0.01). These data indicate that the TFB treatment stimulates protein expression, potentially leading to hyaluronic acid signaling to a significantly greater degree compared with bland moisturizer. TFB, TransFORM Body Treatment.