Figure 4.

Representative IHC staining of tumor TMA and association of FcγRIIB protein expression with PFS for DLBCL patients treated with R-CHOP or G-CHOP in GOYA. TMA sections were stained by IHC for FcγRIIB (brown) and CD68 (red) and assessed by light microscopy. (A) Representative images show ×40 magnification of varying levels of tumor FcγRIIB illustrating negative, weak, medium, and high staining (representative IHC for the Arthur cohort is presented in Arthur et al³⁴). (B) Examples of FcγRIIB on macrophages and corresponding ×63 magnification showing low and high expression. (C) Correlation of FCGR2B expression by RNA-seq with FcγRIIB protein expression on tumor and macrophages. Kaplan-Meier curves for PFS based on FcγRIIB protein expression on (D) tumors* and (E) macrophages.*

*Because of the low number of samples expressing high tumor FcγRIIB, samples expressing high and low FcγRIIB were merged as FcγRIIB⁺ (as opposed to FcγRIIB⁻) to gain greater power for the survival analysis. Similarly, for FcγRIIB expression on macrophages, negative and low samples were merged into 1 group.