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. 2021 Jun 1;88(7):490–499. doi: 10.1002/mrd.23516

Figure 3.

Figure 3

Western blot analysis of phosphorylated MTOR (Ser2448), total MTOR, phosphorylated RPS6K (Thr389), and total RPS6K for blastocyst‐stage embryos cultured without leucine. (a) Representative blots for embryos cultured with a dose curve of GlutaMAX. Target protein molecular weights in kilodaltons (kDa) are depicted to the left of each blot. Alpha‐tubulin was used as the loading control. Densitometry analysis across three replicates (n = 100 embryos per concentration) for (b) phosphorylated MTOR, (c) total MTOR, (d) ratio of phosphorylated to total MTOR, (e) phosphorylated RPS6K, (f) total RPS6K, and (g) ratio of phosphorylated to total RPS6K. Different letters (a,b) indicate statistical differences (p < 0.05). Absence of superscripts above bars indicates that statistical differences were not observed between any of the groups (p > 0.05). RPS6K, ribosomal protein S6 kinase