Figure 5.

Developmental parameters for embryos treated with glutaminase (GLS) inhibitors. (a) Percentage of embryos developing to the blastocyst stage on Day 6 after culture in MU3 (control), MU3 with 100, 250, or 500 µM DON, and MU3 with 1, 5, or 10 µM CB‐839. Values determined across five replicates (n = 250 presumptive zygotes per treatment). (b) Total number of nuclei in Day 6 blastocyst‐stage embryos. Values determined across five replicates (n = 60‐75 embryos per treatment). Data presented as mean ± SEM. Different letters (^a,b,c) indicate statistical differences (p < 0.05). DON, 6‐diazo‐5‐oxo‐l‐norleucine; RPS6K, ribosomal protein S6 kinase