Figure 5.

A) Bright‐field and CLSM images of the interface between hexane and water, along with B) lambda images of stepwise pixel emission in the range of 500–590 nm (increased emission at the interface indicated by arrows). The images represent intensities of emission per pixel at each wavelength indicated in the top‐left corner from excitation at 458 nm (i.e., not donor or acceptor channels). C) Line profile the counts in the 530 nm profile. D) FRET ratio of the donor channel to acceptor channel across the interfacial region. For (C) and (D) the regions are coloured schematically to guide the eye. For all CLSM images the scale bar is 100 μm; all measurements were performed at 24 °C.