Table 1.

Overview of the transformation experiments, T0 generation recovery, and genotyping results.

Method	Round of transformation	Transformed embryos	Plant lines recovered	Plants recovered	Independent T-DNA lines	Plants carrying T-DNA	HvMPK6-mutated lines
Harwood (2014)	1	107	21	26	1	1	1
	2	95	30	39	4	6	0
	3	200	9	21	0	0	0
Total	3	402	60	86	5	7	1 a
Marthe et al. (2015)	1	180	3	3	2	2	0
	2	150	7	7	6	6	0
Total	2	330	10	10	8	8	0
Total both methods	5	732	70	70	13	15	1

^aMutated transgenic line was further designated as line A.