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. 2021 Jul 30;12:670302. doi: 10.3389/fpls.2021.670302

Table 3.

Detection of the on-target mutations and T-DNA in emerged plants in T1, T2, and T3 generation.

Transgenic generation Parental genotype Plants analyzed Plants carrying T-DNA Plants mutated in HvMPK6 WT Mutation frequency % Biallelic Hvmpk6 mutants
T1 Aa (WT/-1T) 37 36 6 (3/3ad) 31 16.22 0
T2 A1 (WT/-1C) 59 59 40 (38/2b) 19 67.80 0
A2 (WT/-1C) 62 62 45 (43/2b) 17 72.58 0
A3 (WT/-1C) 24 24 17c 7 70.83 0
A4a (WT/+1T) 43 43 17 (15/2a) 26 39.53 0
Total sequencing A1+A2+A4 164 164 102 62 62.20 0
Total (WT/-1C) A1+A2+A3 145 145 102 43 70.35 0
Total T2 All 188 188 119 69 63.30 0
T3 A1 (WT/-1C) 40 40 27c 13 67.50 0
A2 (WT/-1C) 20 20 13c 7 65.00 0
Total T3 WT/-1C 60 60 40 c 20 66.67 0
Total (WT/-1C) T2+T3 A1+A2+A3 205 205 142 63 69.27 0
Total T2+T3 All 248 248 159 89 64.11 0

In the column “Plants mutated in HvMPK6,” there are numbers in brackets. The number before slash indicates the number of heterozygotes of the WT HvMPK6 allele and mutant Hvmpk6 allele, the number after slash indicates the number of chimeras.

a

These plants were chimeras composed more of WT cells than of mutated cells.

b

These plants were multiple genotype chimeras of the WT HvMPK6 allele and two different mutated Hvmpk6 alleles.

c

These plants were heterozygotes of the WT HvMPK6 allele and mutant Hvmpk6 allele.

d

These three chimeras include also a chimeric A4 plant, which, in T1 generation, was identified as a putative heterozygous mutant in the HvMPK6 gene (WT/+1T).