Fig. 3.

miR-27a-3p improved the radiosensitivity in HNSCC via targeting SMG1. a Bioinformatic prediction of the binding site between miR-27a-3p and SMG1. The binding site was marked in red. b Dual-luciferase assays were used to detect the luciferase activity in HNSCC cell lines transfected with psiCHECK2, SMG1 WT and SMG1 Mut in the presence of miR-27a-3p mimics. c The relative mRNA expression of SMG1 in HNSCC cell lines treated with miR-27a-3p mimics or inhibitor. d The colonization of TUNEL (+) HNSCC cells under radiation dose of 4 Gy (left panel). The apoptosis rate was evaluated in SMG1 overexpressing HNSCC cell lines treated with or without miR-27a-3p mimics (right panel). e Colony formation assay of SMG1 overexpressing HNSCC cells treated with or without miR-27a-3p mimics (left panel). The number of colonies was counted and compared among different groups (right panel). Data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001