Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The primer sequences used for RT-qPCR
| Gene | Primer |
|---|---|
| HPV16 E6 |
Forward: CTGCAATGTTTCAGGACCCAC Reverse: GTTGTTTGCAGCTCTGTGCAT |
| DGCR8 |
Forward: GTGCATGCTTGTCCCTTTGG Reverse: TGCCAACATACCTCGTAGGG |
| miR-27a-3p |
Forward: CAGTTCACAGTGGCTAAGTTC Reverse: CAGTTTTTTTTTTTTTTTGCGGAA |
| SMG1 |
Forward: AGTTAATGGAGGCCACACCC Reverse: ACTCTAAGGCTTTTACCTTTTTCAA |
| GAPDH |
Forward: AATCCCATCACCATCTTCC Reverse: ATCCGTTGACTCCGACCTTCAC |
| U6 |
Forward: GAGTCCTTCCACGATACCAA Reverse: AACGCT TCACGAATT TGC |
