Table 3.
cif genes in Wolbachia strains of Rhagoletis cerasi
| Gene | Size (aa) | Locus | Orthologue, size (aa), % similarity | Type | Evidence for CI | |
|---|---|---|---|---|---|---|
| wCer1 | INQ25_05555 | 491 | cifA | wHa_RS01435, 492, 91% | I | UNCERTAIN: No crossing experiment data exists; but strong linkage with mitochondrial haplotype (HT1) in Rhagoletis cerasi field populations |
| INQ25_05550 | 1143 | cifB | wHa_RS01430, 1148, 91% | I | ||
| INQ25_01115 | 491 | cifA | wHa_RS01435, 492,88% | I | ||
| INQ25_01120 | 1150 | cifB | wHa_RS01430, 1148, 92% | I | ||
| wCer2 | E3V96_03425 | 475 | cifA | wMel_RS02835, 475, 99% | I | YES: Experimental evidence for CI in multiple novel hosts - Drosophila simulans, Ceratitis capitata, Bactrocera oleae; strong linkage of wCer2 with mitochondrial haplotype (HT2) in R. cerasi field populations |
| E3V96_03430 | 1174 | cifB | wMel_RS06940, 1174, 99.7% | I | ||
| E3V96_02935 | 481 | cifA | wVitA_RS00555, 499, 75% | I | ||
| E3V96_02940 | 1531 | cifB | wVitA_RS00550, 1523, 85% | I | ||
| E3V96_06520 | 492 | cifA | wPip_RS01410, 504, 90% | I | ||
| E3V96_06515 | 921 | cifB | wPip_RS01415, 1175, 83% | I | ||
| E3V96_03725 | 438 | cifA | wIrr_E0495_RS03300, 429, 66% | V | ||
| E3V96_03720 | 3405 | cifB | wStriCN_BVG17_RS00730, 3083, 72% | V | ||
| wCer4 | INQ27_01280 | 494 | cifA | wHa_RS01435, 492, 79% | I | YES: Experimental evidence for strong CI in novel host C. capitata |
| INQ27_01275 | 1166 | cifB | wPip_RS01415, 1175, 85% | I | ||
| INQ27_01270 | 415 | cifA | wStriCN_BVG17_RS06595, 415, 84% | V | ||
| INQ27_01265 | 3332 | cifB | wStriCN_BVG17_RS06590, 4358, 72% | V | ||
| wCer5 | INQ21_01080 | 446 | cifA | wPip_RS01460, 446, 99.8% | IV | UNCERTAIN: No crossing experiment data exists; but very high similarity of cif gene sequences to the Type IV cifA/B genes of wPip with proven CI induction |
| INQ21_01085 | 733 | cifB | wPip_RS01465, 733, 99.6% | IV |