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. 2021 Jul 30;11:678343. doi: 10.3389/fonc.2021.678343

Figure 1.

Figure 1

MTDR inhibits mammosphere formation in MCF7, MDA-MB-231 and MDA-MB-468 cells. (A) Structure of MitoTracker Deep Red FM (MTDR). MTDR is a far-red fluorescent dye that stains active mitochondria. Note that MTDR is a lipophilic cation, which is a chemical characteristic that results its high-efficiency targeting to mitochondria. (B) Effects of MTDR on 3D mammosphere formation in MCF7 cells. Note that MTDR inhibits 3D anchorage-independent growth in MCF7 cells, with an IC-50 of <100 nM. (C) Effects of MTDR on 3D mammosphere formation in MDA-MB-231 cells. Note that MTDR inhibits 3D anchorage-independent growth in MDA-MB-231 cells, with an IC-50 between 100 and 500 nM. (D) Effects of MTDR on 3D mammosphere formation in MDA-MB-468 cells. Note that MTDR inhibits 3D anchorage-independent growth in MDA-MB-468 cells, with an IC-50 between 50 and 100 nM. The statistical test used was a one-tail unpaired t-test.