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. 2021 Jul 30;12:678319. doi: 10.3389/fmicb.2021.678319

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Copyright © 2021 Xu, Chen, Pei, Guo, Li, Yang, Zhang, Yu, Li, Yang, Zhao and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genus composition of a high-complexity artificial microbial community (MOCK2) assessed by shallow WMS or 16S. (A) Genus recall rate. (B) Falsely assigned genus rate by 16S or S-WMS. (C) Stacked bar plot of genera abundances. The values on top of the bar graph indicate the Spearman correlation coefficient between S-WMS/16S data and the actual (Input) values. (D) The relative abundance of dominant genera determined by 16S or S-WMS. The gray bars indicate the actual (Input) value. Sixty-three gut bacterial species (belonging to 28 genera) were cultured under standard laboratory conditions. The artificial community was sequenced five times by each approach. ***p < 0.001.