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Binding of mature and germline IgE antibodies to Fel d 1 by ELISA. Plates were coated with 1 μg/ml Fel d 1 dimer (A), 1 μg/ml Fel d 1 dimer (B), 0.1 μg/ml Fel d 1 dimer (C) and 0.1 μg/ml Fel d 1 monomer (D). Competitive Fel d 1‐IgE binding assay. The IC50 corresponds to the concentration of Fel d 1 dimer at which 50% inhibition of IgE binding to immobilized Fel d 1 is observed (E)
