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The different approaches used to develop in vitro AOI in a crescendo of complexity. The gradient of oxygen was statically engineered by the presence of O2 permeable/impermeable environments (a) or agar‐based gels (b). Differently, the aerobic/anaerobic medium flows allowed modeling AOI in dynamic gut‐on‐a‐chip systems (c). Figure made with biorender (https://biorender.com) [Color figure can be viewed at wileyonlinelibrary.com]
