Reagent	12× amount
10 µM TruSeq‐PE1.0	3.6 µl
10 µM TruSeq‐PE2.0	3.6 µl
Hi‐C library on beads	18 µl
25 mM dNTP mix	3.6 µl
10× Pfu Ultra II Buffer	36 µl
Pfu Ultra II HiFi DNA Polymerase	6 µl
Milli‐Q water	289.2 µl