Sample	Contents
1‐kb ladder (50 ng/µl)	500 ng (10 µl)
	250 ng (5 µl)
	100 ng (2 µl)
	50 ng (1 µl)
Upper Fraction	7 µl sample + 3 µl water + 2 µl 6× Orange G dye
Lower Fraction	3 µl sample + 7 µl water + 2 µl 6× Orange G dye
Lower Fraction	1 µl Hi‐C library + 9 µl water + 2 µl 6× Orange G dye.