Reagent	Amount
10× ligation buffer	7 µl
2.5 mM dNTP mix	7 µl
3 U/µl T4 DNA polymerase	2.5 µl
10 U/µl T4 polynucleotide kinase	2.5 µl
5 U/µl Klenow DNA polymerase	0.5 µl
Milli‐Q water	4.5 µl