Figure 2.

CRISPR‐Cas9‐induced frameshifts in candidate B2‐3 (Rpi‐chc1.1) lead to Phytophthora susceptibility.

(a) Three different sgRNAs were designed to target the LRR domain of candidate B2‐3. (b) A construct with Cas9 and all three sgRNAs was stably transformed into the resistant genotype CHC543‐5. Transformation events were inoculated with P. infestans 90128 and IPO‐C isolates. 48% of the transformation events became susceptible to both isolates (Table S1). (c) One resistant (#1) and three susceptible events (#21, #24, #26) were genotyped together with the untransformed plant CHC543‐5. The three susceptible events contained frameshift mutations, while the tested resistant event had no mutations in the B2‐3 candidate.