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. 2021 May 29;107(1):182–197. doi: 10.1111/tpj.15284

Figure 4.

Figure 4

The RXLR effector PexRD12 corresponds to Avrchc1.1.

(a) The three Rpi‐chc1.1 candidates were co‐agroinfiltrated with the Pex effector collection in N. benthamiana leaves to screen for Avrchc1.1. Rpi‐chc1.1 induces cell death when co‐expressed with both PITG_16233 and PITG_16240, from the PexRD12 family. R3a and Avr3a were used as negative controls, and a mix of R3a and Avr3a was used as a positive control. (b) Relative Avrchc1.1 and Avrsto1 effector expression was measured in infected plant material from a field trial with natural P. infestans infection (2013, Wageningen). Untransformed Desiree plants and Desiree plants transformed with Rpi‐chc1.1 (Desiree:Rpi‐chc1.1) or Rpi‐sto1 (Desiree:Rpi‐sto1) were used in this study. Relative expression data were obtained by dividing the relative effector gene expression by the expression of the P. infestans elongation factor 2 (ef2) gene. Three independent samples were included in the RT‐qPCR experiment. Stars represent a statistically significant difference (two‐sample t‐test, P < 0.008).