TABLE 4.
Distribution of virulence genes in relation to phylogenetic grouping.
| Prevalence of Vfs |
Free-range wool sheep (n = 184)
|
Intensive farming (n = 316)
|
Total (%) (n = 500) | ||||||||||||||||||||||
|
Phylogenetic group
|
Biofilm formation
|
Phylogenetic group
|
Biofilm formation
|
||||||||||||||||||||||
| A | B1 | B2 | C | D | E | F | N | Total | 2 + | 1 + | N | A | B1 | B2 | C | D | E | F | N | Total | 2 + | 1 + | N | ||
| etrA | 2 | 16 | – | 26 | – | 11 | – | 2 | 57 | 2 | 41 | 14 | – | 32 | 1 | 9 | 2 | 1 | – | 4 | 49 | 2 | 41 | 6 | 106(21.2) |
| exhA | 2 | 7 | – | – | – | – | – | 2 | 11 | 2 | 8 | 1 | 3 | 19 | 2 | 7 | 1 | 3 | – | 3 | 38 | 4 | 26 | 8 | 49(9.8) |
| eaeA | 1 | – | – | – | – | – | – | – | 1 | 1 | – | – | – | 1 | – | – | 1 | 1 | – | 2 | 5 | 3 | 2 | – | 6(1.2) |
| fasA, stx1 | – | – | – | – | – | – | – | 1 | 1 | – | 1 | – | – | – | – | – | – | – | – | – | 0 | – | – | – | 1(0.2) |
| sepA, estA | – | – | – | – | – | – | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 1 | 1 | – | 1 | 1(0.2) | |
| hlyA, eaeA | – | – | – | 1 | – | – | – | – | 1 | – | 1 | – | – | 1 | – | – | – | – | – | – | 1 | – | 1 | – | 2(0.4) |
| etrA, exhA | 9 | 38 | – | 6 | – | 5 | – | 2 | 60 | 2 | 45 | 13 | 8 | 60 | – | 20 | 2 | 6 | 2 | 4 | 102 | 7 | 74 | 21 | 162(32.4) |
| etrA, exhA, sfa | 1 | 1 | – | – | – | – | – | 1 | 3 | – | 3 | – | – | 4 | – | – | – | – | – | – | 4 | – | 4 | – | 7(1.4) |
| etrA, exhA, papC | – | – | – | – | – | – | – | 1 | 1 | 1 | – | – | – | 2 | – | 1 | – | – | – | – | 3 | – | 3 | – | 4(0.8) |
| etrA, exhA, aer | 1 | 2 | – | 1 | – | – | – | – | 4 | 1 | 2 | 1 | 3 | 8 | 1 | 2 | – | 3 | 1 | 1 | 19 | 1 | 15 | 3 | 23(4.6) |
| etrA, exhA, faeG | – | 1 | – | – | – | – | 1 | 2 | – | 2 | – | – | – | – | 3 | – | – | – | – | 3 | – | 2 | 1 | 5(1.0) | |
| etrA, exhA, eaeA | 2 | 8 | – | 1 | – | 3 | – | 1 | 15 | 1 | 9 | 5 | 4 | 13 | 1 | 10 | 1 | 2 | – | 6 | 37 | 4 | 28 | 5 | 52(10.4) |
| etrA, exhA, aer, stx2 | 1 | 4 | – | 2 | – | – | – | – | 7 | – | 7 | – | – | 3 | – | – | – | – | – | 1 | 4 | 1 | 2 | 1 | 11(2.2) |
| etrA, exhA, eaeA, cnf1 | – | – | – | – | – | – | – | – | 0 | – | – | – | – | – | – | 1 | – | – | – | 1 | 2 | – | 2 | – | 2(0.4) |
| etrA, exhA, eaeA, fasA | – | 4 | – | – | – | – | – | 2 | 6 | – | 5 | 1 | – | 9 | – | 3 | – | – | – | – | 12 | 1 | 9 | 2 | 18(3.6) |
| etrA, sepA, estA, fasA | – | – | – | 2 | – | 1 | – | – | 3 | – | 3 | – | – | 2 | – | 1 | – | – | – | – | 3 | – | 3 | – | 6(1.2) |
| exhA, eaeA, stx2, hlyA | – | – | – | – | – | – | – | – | 0 | – | – | – | – | – | 1 | – | 1 | – | 2 | – | 2 | – | 2(0.4) | ||
| etrA, exhA, eaeA, fasA, eltA | – | – | – | – | – | – | – | – | 0 | – | – | – | – | – | 1 | – | 1 | – | – | 2 | – | 1 | 1 | 2(0.4) | |
| etrA, exhA, eaeA, fasA, stx1 | – | 1 | – | – | – | – | – | – | 1 | – | 1 | – | – | 1 | – | 1 | – | – | – | – | 2 | – | 2 | 3(0.6) | |
| sfa(1)/hlyA(1)/sepA(2)/aer(4)/faeG(1)/fasA(2)/stx2(1) | – | 1 | – | 2 | – | – | – | 1 | 4 | – | 4 | – | – | 4 | 1 | – | 1 | – | – | 2 | 8 | – | 6 | 2 | 12(2.4) |
| Total no. of isolates with Vfs | 19 | 83 | 0 | 41 | 0 | 20 | 1 | 13 | 177 | 10 | 132 | 35 | 18 | 159 | 6 | 59 | 9 | 17 | 4 | 25 | 297 | 23 | 223 | 51 | 474(94.8) |
| No of isolates without Vfs | – | 1 | – | 1 | – | – | – | 5 | 7 | – | 6 | 1 | – | 10 | 2 | 2 | – | – | – | 5 | 19 | – | 12 | 7 | 26(5.3) |
–, indicates none of the isolates were positive.
N, indicates that isolate strains were not assigned to any phylogenetic group, and biofilm formation.