Table 1.

Semi‐thermal asymmetric reverse PCR (STARP) and Kompetitive allele specific PCR (KASP™) markers developed in this study

Marker	SNP source	Primer type	Primer sequence (5ʹ–3ʹ)	Tm (°C)
rwgsnp6	IWB71956	F ‐ primer 1	[Tail 2]GGAAAACGACGACGACT	54.45
		F ‐ primer 2	[Tail 1]GGAAAACGACGACGCTC	56.07
		R ‐ primer	TGGAAAATCAGCGCTCGACAG	61.27
rwgsnp7	IWB34398	F ‐ primer 1	[Tail 2]AGCACACTACTACGAGAACAT	56.50
		F ‐ primer 2	[Tail 1]AGCACACTACTACGAGACAAG	57.17
		R ‐ primer	CGACCCATACTCAAGACCATCTG	60.49
rwgsnp37.1	CNL13	F ‐ primer 1	[Tail2]AAACCTTTGTTCTCTAACTCTGC	56.99
		F ‐ primer 2	[Tail1]AAACCTTTGTTCTCTAACTACGT	55.83
		R ‐ primer	GCGTCAGCAAGAAGTCATCATCA	61.47
rwgsnp37.2	CNL13	R ‐ primer	CACCATGTATTCAGCAAGAAGTCA	59.30
rwgsnp38	CNL13	F ‐ primer 1	[Tail1]GAATGTATATGTCATGTCCAACG	55.57
		F ‐ primer 2	[Tail2]GAATGTATATGTCATGTCCGCCT	58.43
		R ‐ primer	GCGACTGTAATCTTCAGTTATCCTC	59.31
rwgsnp39	CNL13	F ‐ primer 1	[Tail1]GCCTGAGGAAGTTTAAATATTGG	57.18
		F ‐ primer 2	[Tail2]GCCTGAGGAAGTTTAAATACTAT	56.52
		R ‐ primer	CGTACGCAGAGGATAACTGAAGA	59.94
rwgsnp40	CNL13	F ‐ primer 1	[Tail1] GGAATATACAACCCCATACGAC	56.12
		F ‐ primer 2	[Tail2] GGAATATACAACCCCATATGCG	56.77
		R ‐ primer	CACTTACTTCTTGGCTCAGAAGACA	60.74
KASPSr13	CNL13	F ‐ primer FAM	CACAAAACCTTTGTTCTCTAACTATGC	59.41
		F ‐ primer HEX	CACACAAAACCTTTGTTCTCTAACTATGT	60.63
		R ‐ primer	CCATGTATTCAGCAAGAAGTCATCATCAT	61.99

SNP, single nucleotide polymorphism; T _m, melting temperature.

The primer type in STARP markers: F = forward, R = reverse. STARP markers rwgsnp37.1 and rwgsnp37.2 have the same F primers. For KASP marker KASPSr13, F primer 1 is coupling with FAM for resistant allele C, whereas F primer 2 is coupling with HEX for susceptible allele either T or no amplification.

Primer sequence: Tail1, GCAACAGGAACCAGCTATGAC‐3ʹ; Tail2, GACGCAAGTGAGCAGTATGAC‐3ʹ (Long et al., ²⁰¹⁷).