Fig. 9.

Immunolocalization of crystalline cellulose, xylan, β‐(1 → 4)‐galactan and mannan in phloem fibres in Populus trichocarpa wild‐type (WT), and ptrcesa4, ptrcesa7ab and ptrcesa8ab mutants (CesA, cellulose synthase). Primary and secondary phloem fibers (PPF, SPF) in the 8‐μm transverse cross‐sections of the 20^th internode from each sample were incubated with carbohydrate‐specific antibody (LM10/5/21) or CBM3a‐6 × His protein and anti‐His antibody. Signals (red) were detected with Alexa Fluor 633 goat anti‐rat IgG. CBM3a, LM10, LM5 and LM21 bind crystalline cellulose, xylan, β‐(1 → 4)‐galactan and mannan in plant cell walls, respectively. Bars, 10 μm.