Fig. 7.

DNA methylation-based t-distributed stochastic neighbor embedding distribution. Our 13 tumors were compared to 600 reference samples from the Heidelberg cohort belonging to the HGNET_BCOR (n = 23), HGNET_MN1 (n = 21), EPN_RELA (n = 70), EPN_YAP (n = 11) methylation classes which constitute histopathological differential diagnoses, and EPN-PF_A (n = 91), DMG_K27 (n = 78), GBM_MID (n = 14), GBM_RTK_III (n = 13), GBM_MYCN (n = 16), GBM_MES (n = 56), GBM_RTK_I (n = 64), and GBM_RTK_II (n = 143) and our cases previously reported in Pages et al. of EPN_RELA with proven ZFTA:RELA-fusion (n = 22). The cases in this study, indicated as black dots, were in close proximity to the EPN_RELA subgroup. In a more focused t-SNE analysis of the samples alongside the recently described satellite clusters of ZFTA-fusion positive ependymoma and YAP1-altered ependymoma, four of the cases grouped with cluster 4 and nine with cluster 2