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Intracellular ICD marker and DCs maturation of UCMS@Pep in vitro. A CRT exposure and (B) HMGB1 analysis by immunofluorescence using CLSM (scale bar = 25 μm). Release of (C) ATP and (D) HMGB1 detected by ELISA kits for different interventions. E Schematic of the trans-well DCs maturation system. F Flow cytometry results for mature DCs after different treatments. Levels of cytokines (G) TNF-α and (H) IL-12 in the culture medium. (*P < 0.05, **P < 0.01, ***P < 0.001, n = 3)
