ppGpp coordinates nucleotide and amino-acid synthesis in E. coli during starvation

. Author manuscript; available in PMC: 2021 Oct 1.

Published in final edited form as: Mol Cell. 2020 Aug 27;80(1):29–42.e10. doi: 10.1016/j.molcel.2020.08.005

Strain	Nucleoside	OD₆₀₀ when adding the nucleoside	Treatment time (min)	OD₆₀₀ sampling for
gsk(WT) #ML3272	-	0.02	130	0.25–0.30
	inosine	0.02	130	0.25–0.30
	guanosine	0.02	130	0.25–0.30
gsk(K383A) #ML3272	-	-	-	0.25–0.30
	inosine	0.05	140	0.25–0.30
	guanosine	0.15	130	0.25–0.30
gsk(K383A) pyrE⁺⁺ #ML3273	-	-	-	0.25–0.30
	inosine	0.02	130	0.25–0.30
	guanosine	0.01	130	0.15–0.18