Table 6.
Statistical analysis of the behavioral experiments in P21 and adult females
| Behavioral test | Sex | Age | Normal data? | Equal variance? | Test | Results |
|---|---|---|---|---|---|---|
| Open field test: total distance day 2 | F | P21 | Yes | Yes | One-way ANOVA |
F(2,69) = 9.539; p = 0.0002 Post hoc Tukey’s test: WTSGH vs WTMGH; p = 0.0382 WTSGH vs HET; p = 0.0001 WTMGH vs HET; p = 0.0837 |
| Open field test: total distance day 2 | F | P60 | Yes | Yes | One-way ANOVA |
F(2,69) = 3.990; p = 0.0229 Post hoc Tukey’s test: WTSGH vs WTMGH; p = 0.1094 WTSGH vs HET; p = 0.0214 WTMGH vs HET; p = 0.6459 |
| Open field test: intervals day 2: 0–5 min | F | P21 | No | Yes | Kruskal–Wallis |
H(2) = 21.86; p < 0.0001 Post hoc Dunn’s test: WTSGH vs WTMGH; p = 0.0055 WTSGH vs HET; p < 0.0001 WTMGH vs HET; p = 0.2018 |
| Open field test: intervals day 2: 5–10 min | F | P21 | Yes | Yes | One-way ANOVA |
F(2,69) = 3.290; p = 0.0432 Post hoc Tukey’s test: WTSGH vs WTMGH; p = 0.5888 WTSGH vs HET; p = 0.0359 WTMGH vs HET; p = 0.2036 |
| Open field test: intervals day 2: 10–15 min | F | P21 | Yes | Yes | One-way ANOVA | F(2,69) = 2.102; p = 0.13 |
| Open field test: intervals day 2: 15–20 min | F | P21 | Yes | Yes | One-way ANOVA | F(2,69) = 1.038; p = 0.36 |
| Open field test: intervals day 2: 0–5 min | F | P60 | Yes | Yes | One-way ANOVA |
F(2,69) = 17.95; p < 0.0001 Post hoc Tukey’s test: WTSGH vs WTMGH; p < 0.0001 WTSGH vs HET; p < 0.0001 WTMGH vs HET; p = 0.9276 |
| Open field test: intervals day 2: 5–10 min | F | P60 | Yes | Yes | One-way ANOVA | F(2,69) = 0.228; p = 0.797 |
| Open field test: intervals day 2: 10–15 min | F | P60 | Yes | Yes | One-way ANOVA | F(2,69) = 1.068; p = 0.349 |
| Open field test: intervals day 2: 15–20 min | F | P60 | No | Yes | Kruska–Wallis | H(2) = 3.2334; p = 0.1986 |
| Open field test: time in left day 2 | F | P21 | No | Yes | Kruska–Wallis | H(2) = 4.6819; p = 0.0962 |
| Open field test: time in left day 2 | F | P60 | No | Yes | Kruska–Wallis | H(2) = 4.0863; p = 0.1296 |
| 24 h activity: total | F | >P60 | Yes | Yes | One-way ANOVA | F(2,36) = 1.077; p = 0.3512 |
| 24 h activity: light period | F | >P60 | Yes | Yes | One-way ANOVA | F(2,36) = 2.290; p = 0.1159 |
| 24 h activity: dark period | F | >P60 | Yes | Yes | One-way ANOVA | F(2,36) = 1.103; p = 0.3429 |
| Elevated plus maze | F | P21 | No | Yes | Kruskal–Wallis |
H(2) = 20.943; p < 0.001 Post hoc Dunn’s test: WTSGH vs WTMGH; p = 0.8101 WTSGH vs HET; p = 0.0042 WTMGH vs HET; p < 0.0001 |
| Elevated plus maze | F | P60 | Yes | Yes | One-way ANOVA |
F(2,69) = 5.085; p = 0.0041 Post hoc Tukey’s test: WTSGH vs WTMGH; p = 0.5689 WTSGH vs HET; p = 0.0043 WTMGH vs HET; p = 0.0401 |
| Social interaction | F | P21 | Yes | Yes | One-way ANOVA | F(2,69) = 1.297; p = 0.2425 |
| Social interaction | F | P60 | Yes | Yes | One-way ANOVA |
F(2,69) = 3.536; p = 0.0398 Post hoc Dunnett’s test (all vs WT SGH): WTSGH vs WTMGH; p = 0.0432 WTMGH vs HET; p = 0.9654 |
The table includes the behavioral test analyzed, sex and age of the animals, data and variance distribution, statistical test used, and results obtained. F, female.