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Overexpression and purification of rhGNS utilizing the dhfr gene amplification system. A) The total amount of rhGNS secreted from CHO cells. B) GNS specific activity of secreted rhGNS. C) SDS-PAGE of rhGNS in media and in the myc peptide eluted fraction. In addition to the rhGNS band, the other bands detected in the media sample were CHO proteins released to the media. All activity assays were performed in triplicate, and data are shown as mean ± SD.
