(A) Transcripts shared between diseased and control skin MCs and polyp MCTC (top) vs. enriched in skin MCs (upper middle), polyp MCT (lower middle), or polyp MCTC (bottom) (donor averaged, row normalized).
(B) Representative analysis of MRGPRX2 expression (red) on nasal poly MCs (left) and skin MCs (right) relative to isotype (grey). Staining representative of 3 donors per group.
(C) Identification of MCs based on TPSAB1 expression within a scRNAseq dataset of healthy human lung tissue containing samples from distal, medial and proximal lung generated by Travaglini et al(47).
(D) Violin plots showing expression of the MCTC-associated transcripts CMA1, CTSG, MRGPRX2, and C5AR1 in healthy human lung. No displayed transcripts were significantly differentially expressed between distal and proximal lung, MRGPRX2 expression was only observed in proximal lung.