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. Author manuscript; available in PMC: 2021 Aug 13.
Published in final edited form as: Cell Rep. 2021 Jul 20;36(3):109422. doi: 10.1016/j.celrep.2021.109422

Figure 4. CD8 T cells and B cells act coordinately as lymphoid tissue inducer cells driving TA-TLS development.

Figure 4.

(A, C, and D) Day 14 I.P. B16-OVA tumors from WT mice, μMT−/− mice, Rag1−/− mice, and Rag1−/− mice repleted with CD8 T cells and/or B cells 3 days prior to tumor implantation were prepared for and analyzed by flow cytometry or IF as described in STAR Methods. Scale bars: 100 μm. Data are from one to two experiments; n = 3–10 tumors per group.

(B) CAF from day 14 i.p. tumors from WT or Rag1−/− mice were purified, and expression of the indicated RNA transcripts was determined as described in STAR Methods. Data from two experiments presented as 2−ΔCT relative to Hprt; n = 6 tumors per group.

Results are mean ± SD analyzed using Kruskal-Wallis H test with Dunn’s post-test (A and D) or unpaired Welch’s t test (B and C). nsp > 0.05, *p < 0.05, **p < 0.01, ****p < 0.0001.