Fig 6. Michaelis-Menten plots of enzymatic activity for 6xHis-CBU0823 in relation to malate (A), NAD⁺ (B), and MnCl₂ (C).

Enzyme specific activity (μmol/min per mg protein) measured for (A) varying malate concentrations at 5 mM NAD⁺ and 1mM MnCl₂, (B) varying NAD⁺ concentrations at 3 mM malate and 1 mM MnCl₂, and (C) varying MnCl₂ concentrations at 3 mM malate and 5 mM NAD⁺. Error bars represent standard deviation around the mean from at least three independent experiments.