Fig 5. Acute pan- or endothelial cell-specific deletion of Robo4 was sufficient to alter the location of HSCs.

A. Representative flow cytometry plots of peripheral blood HSCs and multipotent progenitor cells (MPPs). Similar to that of germline R4^KO mice, the numbers of HSCs were significantly elevated in the blood of R4^cKO and R4^ECcKO mice. We have previously published similar results for the R4^KO mice; this panel includes both previously reported and new data from the R4^KO performed side-by-side with the two conditional models [16]. Data represent n = 5–9 independent experiments, with n = wt, n = R4^KO, n = 33 Tamoxifen treated Wt, n = 29 R4^cKO, and n = 18 R4^ECcKO mice. Statistics by unpaired two tailed student’s t-test * p≤0.05, *** p≤0.001. B. Representative flow cytometry plots of BM HSCs and MPPs. C. Significantly fewer HSCs in the BM of R4^KO, R4^cKO and the R4^ECcKO mice as compared to the respective Wt control mice. We have previously published similar results for the R4^KO mice; this panel includes both previously reported [16] and new data from the R4^KO mice performed side-by-side with the two conditional models. Data represent n = 5–9 independent experiments, with n = 25 wt, n = 9 R4^KO, n = 12 Tamoxifen treated Wt, n = 16 R4^cKO, and n = 10 R4^ECcKO mice. Statistics by unpaired two tailed student’s t-test * p≤0.05, *** p≤0.001. See also S3 Fig.