Table 2. SSR loci and the primers used in this study.
Primer ID | Locus | Repeat | Primers (5’-3’) | Number of alleles | Range of Amplicons (bp) |
---|---|---|---|---|---|
P4 | C11CANN1 | (GAT)8(GGT)7 | GTGGTGGTGATGATGATAATGGTGAATTGGTTACGATGGCG | 7 | 233–241 |
P6 | B01-CANN1 | (GAA)13(A)(GAA)3 |
TGGAGTCAAATGAAAGGGAAC
CCATAGCATTATCCCACTCAAG |
11 | 375–387 |
P7 | D02-CANN2 | (CTT)6(ATT)(CTT)10 |
AGATGATGCCAAGAGGCAC
TACCCATCCCCTTGGATCAC |
6 | 120–294 |
P9 | C08-CANN2 | (GA)21 |
GCAAGAAGTAGAGAGACAATCC
CCCTCAACACTCATTAACTCAC |
10 | 236–276 |
P13 | H11CANN1 | (CT)18 |
GCATGTGGTTGTTTCGTACCC
CAGCGAACATTCACTCTAGCTC |
5 | 349–358 |
P14 | B02-CANN2 | (AAG)10 |
CAACCAAATGAGAATGCAACC
TGTTTTCTTCACTGCACCC |
7 | 239–250 |
P15 | H09-CANN2 | (GA)15 |
CGTACAGTGATCGTAGTTGAG
ACACATACAGAGAGAGCCC |
4 | 287–292 |
P17 | E07-CANN1 | (CTA)9 |
CAAATGCCACACCACCTTC
GTAGGTAGCCAGGTATAGGTAG |
5 | 195–201 |
P19 | B05-CANN1 | (TTG)9 |
TTGATGGTGGTGAAACGGC
CCCCAATCTCAATCTCAACCC |
6 | 308–317 |
P24 | D02-CANN1 | (GTT)7 |
GGTTGGGATGTTGTTGTTGTG
AGAAATCCAAGGTCCTGATGG |
4 | 197–202 |
P25 | H06-CANN2 | (ACG)7 |
TGGTTTCAGTGGTCCTCTC
ACGTGAGTGATGACACGAG |
7 | 332–342 |
Primers used were first reported by Alghanim & Almirall [30]. Number of alleles and range of amplicon sizes were obtained from experimental results.