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. 2021 Aug 3;17(8):e1009699. doi: 10.1371/journal.pgen.1009699

Fig 7. OsMADS23 binds to the promoters of OsNCED3, OsNCED4 and OsP5CR, and activates their expression in vivo.

Fig 7

(A) Schematic diagrams of OsNCED3, OsNCED4 and OsP5CR showing the positions of CArG-box motifs and fragments amplified by ChIP-qPCR, respectively. (B) ChIP-qPCR analysis of the gene fragments of OsNCED3, OsNCED4 and OsP5CR enriched by OsMADS23 in rice plants, respectively. The enrichment values were normalized to input. Immunoglobulin G (IgG) immunoprecipitated DNA was used as a control. Ten-day-old rice plants overexpressing OsMADS23-GFP were used for ChIP analysis. Error bars indicate SD with biological triplicates. (C) Schematic diagrams of the effector and reporter used for transient transactivation assays in rice protoplasts. REN, Renilla luciferase; LUC, firefly luciferase. The fragments in the promoter regions of OsNCED3 (-1964 to -1740 bp), OsNCED4, (-1349 to -1100 bp) and OsP5CR (-686 to -781 bp) were used for construction of reporters. (D) Transactivation activity reflected by LUC activity of LUC/REN ratio in rice protoplasts. Error bars indicate SD with biological triplicates. *p < 0.05, **p < 0.01 or ***p < 0.001 (Student’s t test).