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. 2021 Aug 3;17(8):e1009699. doi: 10.1371/journal.pgen.1009699

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© 2021 Li et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 9 — (A) Yeast two-hybrid assays of OsMADS23 and SAPK9. SD, synthetic dropout medium. DDO, SD/-Leu-Trp. QDO, SD/-Ade-His-Leu-Trp. (B) OsMADS23 interacts with SAPK9 in the in vitro pull-down assay. GST-tagged OsMADS23 (GST-OsMADS23) was used as a bait, and pull-down of His-SAPK9 was detected by anti-His antibody (Proteintech). (C) The interaction of OsMADS23 and SAPK9 by bimolecular fluorescence complementation (BiFC) in rice protoplasts. YFP, Yellow fluorescent protein. Scale bars, 5 μm. (D) OsMADS23-SAPK9 interaction detected by coimmunoprecipitation (CoIP) assay. Total proteins from Nicotiana benthamiana leaves coexpressing OsMADS23-GFP with SAPK9-3×FLAG or 3×FLAG were used. Proteins before (input) and after IP were detected by the anti-GFP and anti-FLAG antibodies (Proteintech), respectively. Three independent experiments were performed.