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. Author manuscript; available in PMC: 2021 Aug 13.
Published in final edited form as: Cell Rep. 2020 Dec 22;33(12):108549. doi: 10.1016/j.celrep.2020.108549

Figure 4. Analysis of the Ability of RSMCs to Support Club-Cell Growth In Vitro.

Figure 4.

(A) Experimental setup and gating strategy for cell sorting. Indicated mice were fed tamoxifen-containing food.

(B–G) Overview and representative images of bronchiolospheres.

(H and I) Quantification of organoids.

Scale bars: 100 μm. n = 6 for [Oil/tdTomato+] + [SCGB1A1+], n = 9 for [NA/PDGFRα− tdTomato+] + [SCGB1A1+], and n = 5 for [NA/PDGFRα+ tdTomato+] + [SCGB1A1+]. Data are represented as means ± SEM. *p < 0.05, ****p < 0.0001.