(A) Western blot analysis confirming the loss of AGXT in livers from male Agxt−/−/Apoe−/− mice (n=6).
(B) Glycine to oxalate ratio in plasma from male Agxt−/−/Apoe−/− mice and their Agxt+/+/Apoe−/− littermates (n=9).
(C-G) Male Agxt−/−/Apoe−/− (n=12) and Agxt+/+/Apoe−/− mice (n=10) were fed a WD for 12 weeks: (C) plasma total cholesterol (TC), (D) atherosclerosis in the aortic tree, (E) H&E staining, (F) Oil Red O (ORO) staining, and (G) Mac2 immunohistochemistry of the aortic sinus. (scale bar: 200 μm).
Unpaired t test for B-C and E-G. Mann-Whitney U test for D. Data are presented as mean ± SEM. All points and P values are shown.