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. Author manuscript; available in PMC: 2021 Aug 13.
Published in final edited form as: Mol Cancer Res. 2017 Feb 9;15(6):753–764. doi: 10.1158/1541-7786.MCR-16-0350

Figure 4. TAM expression in MCF10A cells induces chemo-resistance, proliferation, and the activation of Akt; Effects of Gas6 and PS liposomes.

Figure 4

A. Effect of Gas6 and/or Gas6 PS liposomes on camptothecin-mediated cell death in Tyro3-MCF10A cells (A), Axl-MCF10A cells (B) and Mertk-MCF10A cells (C) (see Materials and methods). After 16 hr, cells were evaluated by PI and Annexin V-conjugated FITC to quantify apoptosis. Bar 5 represents a control in which cells were treated in the presence of 50 μM Akt inhibitor LY-294002. D. Effect of Akt inhibitor LY294002 on TAM inducible pAkt. E. Real-time Xcelligence assay to assess cell proliferation induced by Gas6 alone (left panel) and Gas6 with PS liposomes (right panel) on Tyro3-MCF10A, Axl-MCF10A, and Mertk-MCF10A cells. Effect of BMS777607 pretreatment on the Gas6 inducible phosphorylation of Akt (pAkt) and phosphorylation of TAMs; pTyro3 (F), pAxl (G) and pMertk (H) is shown.