Reagent	Amount
Nuclease‐free water	x µl
Purified custom 5′sgRNA (e.g.NBS1 sgRNA‐B) (step 37)	x µl (16 µg)
Purified custom 3′sgRNA (e.g., NBS1 sgRNA‐C) (step 37)	x µl (16 µg)
10× Reaction Buffer 3.1	20 µl
Cas9 Nuclease (20 µM)	4 µl
Purified genomic DNA (step 57)	x µl (5 µg)
Total reaction volume	100 µl